Our histology services currently include:
- Histology and histochemistry
- Immunohistochemistry
- In-situ hybridization
- Consultative services
Specimen grossing and microscopy are performed at both our main and Centralia
offices; specimen processing (cutting and staining) and reporting take place in
the main office.
Between our two facilities we employ five histotechnicians, one
histotechnician-in-training, three histology assistants, and two fellowship
employees.
Supplies
We provide fixative, requisitions, labels and specimen bags for our clients,
free of charge. Place an order for supplies via our online
order
form, or use the contact numbers below.
Laboratory Hours
Regular laboratory hours are 8 a.m. -- 5 p.m.
Contact Information
Specimen Preparation
Please refer to our procedure guide for proper tissue submission instructions
Histology
Glossary
| Fixative |
A chemical that
must do everything a preservative does, plus modify tissue constituents in
such a way that they retain their form when subjected to treatment that
would damage them in their initial state. |
| Fixative vs. Tissue Ratio |
The amount of fixative
compared to the volume or size of the tissue specimen.
(Fixative fluid should be 15 -20 times the volume of tissue
specimen.) |
| Accessioning |
Verifying patient
information; identifying type of tissue specimen; dating and assigning lab
identification number. |
| Grossing |
Pathologist
dictates the description of appearance of tissue specimen.
Size, shape, color, and texture of tissue received.
They then take a sampling of the specimen for processing. |
A Biopsy's Journey Through the Histology Laboratory
-
Patient is biopsied--tissue is placed in fixative solution.
-
Specimen is brought to the lab by the courier service.
-
Histotechnologist or assistant identifies, accessions and
records the tissue.
-
Pathologist selects a portion of the specimen for histologic
processing and diagnosis.
-
The biopsy tissue sample is preserved in proper chemical
fixative.
-
Tissue processing:
-
Fixation, Dehydration, Clearing, Infiltration--takes
place in a tissue processor. The process takes approximately 12-15 hours
and is completed overnight.
-
The tissue is embedded in paraffin.
-
The paraffin block containing tissue is cut on a microtome
in microthin slices and mounted on a glass slide.
-
Routine staining is performed and then they are
coverslipped.
-
Non-routine staining is performed on some specimens for:
-
specific tissue structures
-
metabolic by-products
-
specific cellular morphology
infectious microorganisms -
endogenous and/or exogenous pigments
-
The pathologist receives the stained slides for
interpretation and diagnosis.
-
Transcriptionists type and generate a pathology report from
pathologist findings.
Visit our FAQ page for answers to
common questions.
Are you in need of anything from us? What do you think about our
service, services or web site? Visit our
feedback
form and let us know!
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